› 2anj

X-ray diffraction
2.1Å resolution

Crystal Structure of the Glur2 Ligand Binding Core (S1S2J-Y450W) Mutant in Complex With the Partial Agonist Kainic Acid at 2.1 A Resolution

Released:
DOI: 10.2210/pdb2anj/pdb
PDB entry 2anj coloured by chain, front view.
PDB entry 2anj coloured by chain, side view.
PDB entry 2anj coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A binding site tyrosine shapes desensitization kinetics and agonist potency at GluR2. A mutagenic, kinetic, and crystallographic study.
Holm MM, Naur P, Vestergaard B, Geballe MT, Gajhede M, Kastrup JS, Traynelis SF, Egebjerg J
J Biol Chem 280 35469-76 (2005)
PMID: 16103115

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand KAI 1 x KAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21212
Unit cell:
a: 95.254Å b: 61.635Å c: 48.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Pharmacological insights obtained from structure-function studies of ionotropic glutamate receptors.
Chen et al. (2006)
3 more