› 2ass

X-ray diffraction
3Å resolution

Crystal structure of the Skp1-Skp2-Cks1 complex

Released:
DOI: 10.2210/pdb2ass/pdb
PDB entry 2ass coloured by chain, front view.
PDB entry 2ass coloured by chain, side view.
PDB entry 2ass coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase.
Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP
Mol Cell 20 9-19 (2005)
PMID: 16209941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-158185 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 2-160; Coverage: 98%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
S-phase kinase-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13309 (Residues: 89-424; Coverage: 79%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Structure domains: Ribonuclease Inhibitor
Cyclin-dependent kinases regulatory subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 69 amino acids
Theoretical weight: 8.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61024 (Residues: 5-73; Coverage: 87%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cyclin-dependent kinase, regulatory subunit

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand BEN 2 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3221
Unit cell:
a: 149.332Å b: 149.332Å c: 99.916Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

46 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
45 more

15 mentions without citation

Targeting Cullin-RING E3 ubiquitin ligases for drug discovery: structure, assembly and small-molecule modulation.
Bulatov et al. (2015)
14 more