› 2auw

X-ray diffraction
1.85Å resolution

Crystal Structure of Putative DNA Binding Protein NE0471 from Nitrosomonas europaea ATCC 19718

Released:
DOI: 10.2210/pdb2auw/pdb
PDB entry 2auw coloured by chain, front view.
PDB entry 2auw coloured by chain, side view.
PDB entry 2auw coloured by chain, top view.
Source organism: Nitrosomonas europaea
Entry authors: Kim Y, Joachimiak A, Skarina T, Savchenko A, Edwards A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF2442 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.38 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82X29 (Residues: 1-166; Coverage: 100%)
Gene name: NE0471
Sequence domains: Protein of unknown function (DUF2442)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand FMT 1 x FMT
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 54.13Å b: 157.565Å c: 85.707Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.197 0.245
Expression system: Escherichia coli BL21(DE3)

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