› 2awi

X-ray diffraction
2.25Å resolution

Structure of PrgX Y153C mutant

Released:
DOI: 10.2210/pdb2awi/pdb
PDB entry 2awi coloured by chain, front view.
PDB entry 2awi coloured by chain, side view.
PDB entry 2awi coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis.
Shi K, Brown CK, Gu ZY, Kozlowicz BK, Dunny GM, Ohlendorf DH, Earhart CA
Proc Natl Acad Sci U S A 102 18596-601 (2005)
PMID: 16339309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 317 amino acids
Theoretical weight: 37.27 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04114 (Residues: 1-317; Coverage: 100%)
Gene name: prgX
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P21
Unit cell:
a: 92.527Å b: 134.366Å c: 195.199Å
α: 90° β: 100.28° γ: 90°
R-values:
R R work R free
0.221 0.218 0.271
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Antibiotic resistant enterococci-tales of a drug resistance gene trafficker.
Werner et al. (2013)
16 more

2 mentions without citation

Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis.
Shi et al. (2005)
1 more