› 2axz

X-ray diffraction
3Å resolution

Crystal structure of PrgX/cCF10 complex

Released:
DOI: 10.2210/pdb2axz/pdb
PDB entry 2axz coloured by chain, front view.
PDB entry 2axz coloured by chain, side view.
PDB entry 2axz coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis.
Shi K, Brown CK, Gu ZY, Kozlowicz BK, Dunny GM, Ohlendorf DH, Earhart CA
Proc Natl Acad Sci U S A 102 18596-601 (2005)
PMID: 16339309

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero heptamer
Assembly name:
PDBe Complex ID:
PDB-CPX-162636 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 37.42 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04114 (Residues: 1-317; Coverage: 100%)
Gene name: prgX
Sequence domains:
Structure domains:
LVTLVFV peptide Chains: E, F, H
Molecule details ›
Chains: E, F, H
Length: 7 amino acids
Theoretical weight: 790 Da
Source organism: Enterococcus faecalis
Expression system: Not provided
TPPKEVT(MSE) peptide Chain: I
Molecule details ›
Chain: I
Length: 8 amino acids
Theoretical weight: 950 Da
Source organism: Enterococcus faecalis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 25 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 71.076Å b: 83.903Å c: 286.804Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Antibiotic resistant enterococci-tales of a drug resistance gene trafficker.
Werner et al. (2013)
15 more

7 mentions without citation

Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.
Do et al. (2016)
6 more