› 2ayh

X-ray diffraction
1.6Å resolution

CRYSTAL AND MOLECULAR STRUCTURE AT 1.6 ANGSTROMS RESOLUTION OF THE HYBRID BACILLUS ENDO-1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE H(A16-M)

Released:
DOI: 10.2210/pdb2ayh/pdb
PDB entry 2ayh coloured by chain, front view.
PDB entry 2ayh coloured by chain, side view.
PDB entry 2ayh coloured by chain, top view.
Source organism: hybrid
Primary publication:
Crystal and molecular structure at 0.16-nm resolution of the hybrid Bacillus endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase H(A16-M).
Hahn M, Keitel T, Heinemann U
Eur. J. Biochem. 232 849-58 (1995)
PMID: 7588726

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.94 KDa
Source organism: hybrid
Expression system: Escherichia coli
UniProt:
  • Canonical: P23904 (Residues: 25-237; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.18Å b: 78.26Å c: 39.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 not available not available
Expression system: Escherichia coli

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Citations

3 review citations

Microbial hydrolysis of polysaccharides.
Warren RA. (1996)
2 more

18 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
17 more