› 2b22

X-ray diffraction
2Å resolution

Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat

Released:
DOI: 10.2210/pdb2b22/pdb
PDB entry 2b22 coloured by chain, front view.
PDB entry 2b22 coloured by chain, side view.
PDB entry 2b22 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat.
Deng Y, Liu J, Zheng Q, Eliezer D, Kallenbach NR, Lu M
Structure 14 247-55 (2006)
PMID: 16472744

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chain: A
Molecule details ›
Chain: A
Length: 34 amino acids
Theoretical weight: 3.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 251-281; Coverage: 11%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I4122
Unit cell:
a: 45.985Å b: 45.985Å c: 52.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.224 0.276
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Fifty years of coiled-coils and alpha-helical bundles: a close relationship between sequence and structure.
Parry et al. (2008)
3 more

7 mentions without citation

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)
6 more