› 2b4o

X-ray diffraction
2.3Å resolution

Structure of the R258K mutant of Selenomonas ruminantium PTP-like phytase

Released:
DOI: 10.2210/pdb2b4o/pdb
PDB entry 2b4o coloured by chain, front view.
PDB entry 2b4o coloured by chain, side view.
PDB entry 2b4o coloured by chain, top view.
Source organism: Selenomonas ruminantium
Primary publication:
Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase.
Puhl AA, Gruninger RJ, Greiner R, Janzen TW, Mosimann SC, Selinger LB
Protein Sci 16 1368-78 (2007)
PMID: 17567745

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1L-myo-inositol 1,2,3,4,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 38.33 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WUJ1 (Residues: 34-346; Coverage: 98%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 6 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 45.837Å b: 137.187Å c: 79.954Å
α: 90° β: 103.53° γ: 90°
R-values:
R R work R free
0.191 0.191 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Phytase, a new life for an "old" enzyme.
Lei et al. (2013)
3 more

1 mention without citation

Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase.
Puhl et al. (2007)