› 2b8s

X-ray diffraction
2.76Å resolution

Structure of HIV-1(MAL) genomic RNA DIS

Released:
DOI: 10.2210/pdb2b8s/pdb
PDB entry 2b8s coloured by chain, front view.
PDB entry 2b8s coloured by chain, side view.
PDB entry 2b8s coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structures of coaxially stacked kissing complexes of the HIV-1 RNA dimerization initiation site.
Ennifar E, Walter P, Ehresmann B, Ehresmann C, Dumas P
Nat Struct Biol 8 1064-8 (2001)
PMID: 11702070

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196317 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*CP*UP*UP*GP*CP*UP*GP*AP*GP*GP*UP*GP*CP*AP*CP*AP*CP*AP*GP*CP*AP*AP*G)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 23 nucleotides
Theoretical weight: 7.4 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 6 x MG
Ligand SO4 6 x SO4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2221
Unit cell:
a: 27.076Å b: 114.822Å c: 94.399Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.213
Expression system: Not provided

Quick links

Citations

26 review citations

Ions and RNA folding.
Draper et al. (2005)
25 more

6 mentions without citation

Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions.
Bou-Nader et al. (2020)
5 more