› 2ba9

X-ray diffraction
1.95Å resolution

Crystal structure of CLA-producing fatty acid isomerase from P. acnes

Released:
DOI: 10.2210/pdb2ba9/pdb
PDB entry 2ba9 coloured by chain, front view.
PDB entry 2ba9 coloured by chain, side view.
PDB entry 2ba9 coloured by chain, top view.
Source organism: Cutibacterium acnes
Primary publication:
Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase.
Liavonchanka A, Hornung E, Feussner I, Rudolph MG
Proc Natl Acad Sci U S A 103 2576-81 (2006)
PMID: 16477020

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amine oxidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 49.08 KDa
Source organism: Cutibacterium acnes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6A8X5 (Residues: 1-424; Coverage: 100%)
Gene name: PPA1039
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand FAD 1 x FAD
Ligand 12P 1 x 12P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 133.707Å b: 60.759Å c: 72.158Å
α: 90° β: 115.85° γ: 90°
R-values:
R R work R free
0.215 0.213 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Board-invited review: Recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem.
Jenkins et al. (2008)
8 more

2 mentions without citation

Microbial Production of Conjugated Linoleic Acid and Conjugated Linolenic Acid Relies on a Multienzymatic System.
Salsinha et al. (2018)
1 more