› 2bak

X-ray diffraction
2.2Å resolution

p38alpha MAP kinase bound to MPAQ

Released:
DOI: 10.2210/pdb2bak/pdb
PDB entry 2bak coloured by chain, front view.
PDB entry 2bak coloured by chain, side view.
PDB entry 2bak coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Prevention of MKK6-dependent activation by binding to p38alpha MAP kinase.
Sullivan JE, Holdgate GA, Campbell D, Timms D, Gerhardt S, Breed J, Breeze AL, Bermingham A, Pauptit RA, Norman RA, Embrey KJ, Read J, VanScyoc WS, Ward WH
Biochemistry 44 16475-90 (2005)
PMID: 16342939

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 42.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16539 (Residues: 2-360; Coverage: 100%)
Gene names: CSBP, CSBP1, CSBP2, CSPB1, MAPK14, MXI2, SAPK2A
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AQZ 1 x AQZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 66.332Å b: 75.84Å c: 78.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.25
Expression system: Escherichia coli

Quick links

Citations

6 review citations

p38 pathway kinases as anti-inflammatory drug targets.
Schindler et al. (2007)
5 more

9 mentions without citation

Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock.
Bikadi et al. (2009)
8 more