› 2bdo

Solution NMR

SOLUTION STRUCTURE OF HOLO-BIOTINYL DOMAIN FROM ACETYL COENZYME A CARBOXYLASE OF ESCHERICHIA COLI DETERMINED BY TRIPLE-RESONANCE NMR SPECTROSCOPY

Released:
DOI: 10.2210/pdb2bdo/pdb
PDB entry 2bdo coloured by chain, ensemble of 23 models, front view.
PDB entry 2bdo coloured by chain, ensemble of 23 models, side view.
PDB entry 2bdo coloured by chain, ensemble of 23 models, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Solution structures of apo and holo biotinyl domains from acetyl coenzyme A carboxylase of Escherichia coli determined by triple-resonance nuclear magnetic resonance spectroscopy.
Roberts EL, Shu N, Howard MJ, Broadhurst RW, Chapman-Smith A, Wallace JC, Morris T, Cronan JE, Perham RN
Biochemistry 38 5045-53 (1999)
PMID: 10213607

Function and Biology Details

Reaction catalysed: 
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin carboxyl carrier protein of acetyl-CoA carboxylase Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.7 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABD8 (Residues: 77-156; Coverage: 51%)
Gene names: JW3223, accB, b3255, fabE
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments


Cofactor: Ligand BTN 1 x BTN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Is allostery an intrinsic property of all dynamic proteins?
Gunasekaran et al. (2004)
10 more

2 mentions without citation

The Classical, Yet Controversial, First Enzyme of Lipid Synthesis: Escherichia coli Acetyl-CoA Carboxylase.
Cronan JE. (2021)
1 more