› 2bep

X-ray diffraction
1.8Å resolution

Crystal structure of ubiquitin conjugating enzyme E2-25K

Released:
DOI: 10.2210/pdb2bep/pdb
PDB entry 2bep coloured by chain, front view.
PDB entry 2bep coloured by chain, side view.
PDB entry 2bep coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
SUMO modification of the ubiquitin-conjugating enzyme E2-25K.
Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Körner R, Olsen JV, Jentsch S, Melchior F, Sixma TK
Nat Struct Mol Biol 12 264-9 (2005)
PMID: 15723079

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 K Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.85 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61085 (Residues: 1-155; Coverage: 78%)
Gene names: HIP2, UBE2K
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 38.256Å b: 68.153Å c: 78.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Concepts in sumoylation: a decade on.
Geiss-Friedlander et al. (2007)
28 more

2 mentions without citation

Structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2).
Wilson et al. (2009)
1 more