› 2bhl

X-ray diffraction
2.9Å resolution

X-RAY STRUCTURE OF HUMAN GLUCOSE-6-PHOSPHATE DEHYDROGENASE (DELETION VARIANT) COMPLEXED WITH GLUCOSE-6-PHOSPHATE

Released:
DOI: 10.2210/pdb2bhl/pdb
PDB entry 2bhl coloured by chain, front view.
PDB entry 2bhl coloured by chain, side view.
PDB entry 2bhl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of glucose-6-phosphate and NADP+ binding to human glucose-6-phosphate dehydrogenase.
Kotaka M, Gover S, Vandeputte-Rutten L, Au SW, Lam VM, Adams MJ
Acta Crystallogr D Biol Crystallogr 61 495-504 (2005)
PMID: 15858258

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 56.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11413 (Residues: 28-515; Coverage: 95%)
Gene name: G6PD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand BG6 2 x BG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2221
Unit cell:
a: 117.552Å b: 179.527Å c: 137.938Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.261
Expression system: Escherichia coli

Quick links

Citations

8 review citations

G6PD deficiency: the genotype-phenotype association.
Mason et al. (2007)
7 more

37 mentions without citation

Glucose-6-Phosphate Dehydrogenase: Update and Analysis of New Mutations around the World.
Gómez-Manzo et al. (2016)
36 more