› 2bi3

X-ray diffraction
1.69Å resolution

Radiation damage of the Schiff base in phosphoserine aminotransferase (structure D)

Released:
DOI: 10.2210/pdb2bi3/pdb
PDB entry 2bi3 coloured by chain, front view.
PDB entry 2bi3 coloured by chain, side view.
PDB entry 2bi3 coloured by chain, top view.
Source organism: Alkalihalobacillus alcalophilus
Primary publication:
Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.
Dubnovitsky AP, Ravelli RB, Popov AN, Papageorgiou AC
Protein Sci 14 1498-507 (2005)
PMID: 15883191

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 40.24 KDa
Source organism: Alkalihalobacillus alcalophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RME2 (Residues: 2-361; Coverage: 100%)
Gene name: serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
4 bound ligands:
Ligand MG 4 x MG
Ligand CL 4 x CL
Ligand 1PE 1 x 1PE
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 143.061Å b: 83.772Å c: 66.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 not available 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
2 more

1 mention without citation

Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.
Dubnovitsky et al. (2005)