› 2bk0

X-ray diffraction
2.9Å resolution

Crystal structure of the major celery allergen Api G 1

Released:
DOI: 10.2210/pdb2bk0/pdb
PDB entry 2bk0 coloured by chain, front view.
PDB entry 2bk0 coloured by chain, side view.
PDB entry 2bk0 coloured by chain, top view.
Source organism: Apium graveolens
Primary publication:
Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity.
Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Marković-Housley Z
J Mol Biol 351 1101-9 (2005)
PMID: 16051263

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major allergen Api g 1, isoallergen 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 16.33 KDa
Source organism: Apium graveolens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49372 (Residues: 1-154; Coverage: 100%)
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ROTATING GENERATOR
Spacegroup: C2
Unit cell:
a: 105.878Å b: 67.947Å c: 48.001Å
α: 90° β: 91.18° γ: 90°
R-values:
R R work R free
0.224 0.221 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structural and functional aspects of PR-10 proteins.
Fernandes et al. (2013)
5 more

16 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
15 more