› 2blp

X-ray diffraction
1.4Å resolution

RNase before unattenuated X-RAY burn

Released:
DOI: 10.2210/pdb2blp/pdb
PDB entry 2blp coloured by chain, front view.
PDB entry 2blp coloured by chain, side view.
PDB entry 2blp coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Improving radiation-damage substructures for RIP.
Nanao MH, Sheldrick GM, Ravelli RB
Acta Crystallogr D Biol Crystallogr 61 1227-37 (2005)
PMID: 16131756

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 64.132Å b: 64.132Å c: 63.643Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.145 0.176

Quick links

Citations

2 review citations

Synergy within structural biology of single crystal optical spectroscopy and X-ray crystallography.
De la Mora-Rey et al. (2007)
1 more

4 mentions without citation

Identifying and quantifying radiation damage at the atomic level.
Gerstel et al. (2015)
3 more