› 2bnn

X-ray diffraction
2.5Å resolution

The structure of Hydroxypropylphosphonic acid epoxidase from S. wedmorenis in complex with fosfomycin

Released:
DOI: 10.2210/pdb2bnn/pdb
PDB entry 2bnn coloured by chain, front view.
PDB entry 2bnn coloured by chain, side view.
PDB entry 2bnn coloured by chain, top view.
Source organism: Streptomyces wedmorensis
Primary publication:
Structure and reactivity of hydroxypropylphosphonic acid epoxidase in fosfomycin biosynthesis by a cation- and flavin-dependent mechanism.
McLuskey K, Cameron S, Hammerschmidt F, Hunter WN
Proc Natl Acad Sci U S A 102 14221-6 (2005)
PMID: 16186494

Function and Biology Details

Reaction catalysed: 
(S)-2-hydroxypropylphosphonate + H(2)O(2) = (1R,2S)-1,2-epoxypropylphosphonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-hydroxypropylphosphonic acid epoxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 21.36 KDa
Source organism: Streptomyces wedmorensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56185 (Residues: 1-198; Coverage: 100%)
Gene names: fom4, hppE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FCN 2 x FCN
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6522
Unit cell:
a: 85.602Å b: 85.602Å c: 217.989Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.184 0.27
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
5 more

2 mentions without citation

Structure and reactivity of hydroxypropylphosphonic acid epoxidase in fosfomycin biosynthesis by a cation- and flavin-dependent mechanism.
McLuskey et al. (2005)
1 more