› 2bnr

X-ray diffraction
1.9Å resolution

Structural and kinetic basis for heightened immunogenicity of T cell vaccines

Released:
DOI: 10.2210/pdb2bnr/pdb
PDB entry 2bnr coloured by chain, front view.
PDB entry 2bnr coloured by chain, side view.
PDB entry 2bnr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic basis for heightened immunogenicity of T cell vaccines.
Chen JL, Stewart-Jones G, Bossi G, Lissin NM, Wooldridge L, Choi EM, Held G, Dunbar PR, Esnouf RM, Sami M, Boulter JM, Rizkallah P, Renner C, Sewell A, van der Merwe PA, Jakobsen BK, Griffiths G, Jones EY, Cerundolo V
J Exp Med 201 1243-55 (2005)
PMID: 15837811

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100941 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 157-165; Coverage: 5%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
T cell receptor alpha chain constant; T cell receptor alpha variable 21 Chain: D
Molecule details ›
Chain: D
Length: 203 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J279 (Residues: 21-112; Coverage: 99%)
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC, TRAV21
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1; T cell receptor beta variable 6-5 Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K0K1A5 (Residues: 22-114; Coverage: 100%)
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene names: TRBC1, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 72.563Å b: 62.49Å c: 116.955Å
α: 90° β: 105.34° γ: 90°
R-values:
R R work R free
0.231 0.231 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
30 more

38 mentions without citation

Structure-Based, Rational Design of T Cell Receptors.
Zoete et al. (2013)
37 more