› 2bnz

X-ray diffraction
2.6Å resolution

Structural basis for cooperative binding of Ribbon-Helix-Helix Omega repressor to inverted DNA heptad repeats

Released:
DOI: 10.2210/pdb2bnz/pdb
PDB entry 2bnz coloured by chain, front view.
PDB entry 2bnz coloured by chain, side view.
PDB entry 2bnz coloured by chain, top view.
Source organisms:
Primary publication:
Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription.
Weihofen WA, Cicek A, Pratto F, Alonso JC, Saenger W
Nucleic Acids Res 34 1450-8 (2006)
PMID: 16528102

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115118 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Omega transcriptional repressor domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 53 amino acids
Theoretical weight: 6.14 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q57468 (Residues: 20-71; Coverage: 73%)
Gene name: omega
Sequence domains: Omega Transcriptional Repressor
Structure domains: Met repressor-like
5'-D(*GP*AP*AP*TP*CP*AP*CP*AP*AP*GP *TP*GP*AP*TP*TP*AP*GP*C)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 18 nucleotides
Theoretical weight: 5.55 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*CP*TP*AP*AP*TP*CP*AP*CP*TP*TP *GP*TP*GP*AP*TP*TP*CP*G)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 75.991Å b: 42.505Å c: 103.727Å
α: 90° β: 107.17° γ: 90°
R-values:
R R work R free
0.227 0.225 0.258
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

8 review citations

To be or not to be: regulation of restriction-modification systems and other toxin-antitoxin systems.
Mruk et al. (2014)
7 more

4 mentions without citation

Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription.
Weihofen et al. (2006)
3 more