› 2brt

X-ray diffraction
2.2Å resolution

ANTHOCYANIDIN SYNTHASE FROM ARABIDOPSIS THALIANA COMPLEXED with naringenin

Released:
DOI: 10.2210/pdb2brt/pdb
PDB entry 2brt coloured by chain, front view.
PDB entry 2brt coloured by chain, side view.
PDB entry 2brt coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism.
Welford RW, Clifton IJ, Turnbull JJ, Wilson SC, Schofield CJ
Org Biomol Chem 3 3117-26 (2005)
PMID: 16106293

Function and Biology Details

Reaction catalysed: 
(1a) a (2R,3S,4S)-leucoanthocyanidin + 2-oxoglutarate + O(2) = a (4S)- 2,3-dehydroflavan-3,4-diol + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucoanthocyanidin dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96323 (Residues: 2-356; Coverage: 100%)
Gene names: At4g22880, F7H19.60, LDOX
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

3 bound ligands:
Ligand FE2 1 x FE2
Ligand AKG 1 x AKG
Ligand NAR 1 x NAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 56.807Å b: 62.309Å c: 102.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The evolution of phenylpropanoid metabolism in the green lineage.
Tohge et al. (2013)
4 more

10 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
9 more