› 2bti

X-ray diffraction
2Å resolution

Structure-function studies of the RmsA CsrA post-transcriptional global regulator protein family reveals a class of RNA-binding structure

Released:
DOI: 10.2210/pdb2bti/pdb
PDB entry 2bti coloured by chain, front view.
PDB entry 2bti coloured by chain, side view.
PDB entry 2bti coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
Functional analysis of the post-transcriptional regulator RsmA reveals a novel RNA-binding site.
Heeb S, Kuehne SA, Bycroft M, Crivii S, Allen MD, Haas D, Cámara M, Williams P
J Mol Biol 355 1026-36 (2006)
PMID: 16359708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106619 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translational regulator CsrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.09 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1JK11 (Residues: 1-61; Coverage: 100%)
Gene names: YE0835, csrA, rsmA
Sequence domains: Global regulator protein family

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P4322
Unit cell:
a: 37.573Å b: 37.573Å c: 186.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.254
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Gac/Rsm signal transduction pathway of gamma-proteobacteria: from RNA recognition to regulation of social behaviour.
Lapouge et al. (2008)
10 more

5 mentions without citation

Simultaneous prediction of protein folding and docking at high resolution.
Das et al. (2009)
4 more