› 2buj

X-ray diffraction
2.6Å resolution

Crystal structure of the human Serine-threonine Kinase 16 in complex with staurosporine

Released:
DOI: 10.2210/pdb2buj/pdb
PDB entry 2buj coloured by chain, front view.
PDB entry 2buj coloured by chain, side view.
PDB entry 2buj coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Debreczeni JE, Eswaran J, Bullock A, Filippakopoulos P, Kavanagh K, Amos A, Fedorov O, Sobott F, Ball LJ, von Delft F, Arrowsmith C, Sundstrom M, Edwards A, Knapp S

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase 16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O75716 (Residues: 13-305; Coverage: 96%)
Gene names: MPSK1, PKL12, STK16, TSF1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand STU 4 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 104.554Å b: 168.901Å c: 129.729Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.229
Expression system: Escherichia coli BL21

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