› 2bvw

X-ray diffraction
1.7Å resolution

CELLOBIOHYDROLASE II (CEL6A) FROM HUMICOLA INSOLENS IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE

Released:
DOI: 10.2210/pdb2bvw/pdb
PDB entry 2bvw coloured by chain, front view.
PDB entry 2bvw coloured by chain, side view.
PDB entry 2bvw coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Structural changes of the active site tunnel of Humicola insolens cellobiohydrolase, Cel6A, upon oligosaccharide binding.
Varrot A, Schülein M, Davies GJ
Biochemistry 38 8884-91 (1999)
PMID: 10413461

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Exoglucanase-6A Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 40.01 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q9C1S9 (Residues: 115-476; Coverage: 79%)
Gene names: avi2, cel6A
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand BGC 2 x BGC
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 48.56Å b: 154.43Å c: 51.04Å
α: 90° β: 119.31° γ: 90°
R-values:
R R work R free
0.175 0.175 0.226
Expression system: Aspergillus oryzae

Quick links

Citations

4 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
3 more

3 mentions without citation

Crystal structures of the catalytic domain of a novel glycohydrolase family 23 chitinase from Ralstonia sp. A-471 reveals a unique arrangement of the catalytic residues for inverting chitin hydrolysis.
Arimori et al. (2013)
2 more