› 2bwc

X-ray diffraction
2.15Å resolution

Structure of Endoglucanase 12A (Cel12A) from Rhodothermus marinus in complex with cellopentaose (5 minute soak)

Released:
DOI: 10.2210/pdb2bwc/pdb
PDB entry 2bwc coloured by chain, front view.
PDB entry 2bwc coloured by chain, side view.
PDB entry 2bwc coloured by chain, top view.
Source organism: Rhodothermus marinus
Primary publication:
Dimerisation and an increase in active site aromatic groups as adaptations to high temperatures: X-ray solution scattering and substrate-bound crystal structures of Rhodothermus marinus endoglucanase Cel12A.
Crennell SJ, Cook D, Minns A, Svergun D, Andersen RL, Nordberg Karlsson E
J Mol Biol 356 57-71 (2006)
PMID: 16343530

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cellulase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.21 KDa
Source organism: Rhodothermus marinus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33897 (Residues: 38-126, 127-128, 129-260; Coverage: 84%)
Gene name: celA
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
3 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 1 x SO4
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 55.89Å b: 67.33Å c: 131.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural NMR of protein oligomers using hybrid methods.
Wang et al. (2011)
12 other articles

2 mentions without citation

Structure and stability of metagenome-derived glycoside hydrolase family 12 cellulase (LC-CelA) a homolog of Cel12A from Rhodothermus marinus.
Okano et al. (2014)
1 more