› 2bxj

X-ray diffraction
2.4Å resolution

Double Mutant of the Ribosomal Protein S6

Released:
DOI: 10.2210/pdb2bxj/pdb
PDB entry 2bxj coloured by chain, front view.
PDB entry 2bxj coloured by chain, side view.
PDB entry 2bxj coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Antagonism, non-native interactions and non-two-state folding in S6 revealed by double-mutant cycle analysis.
Otzen D
Protein Eng Des Sel 18 547-57 (2005)
PMID: 16230299

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit protein bS6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.9 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23370 (Residues: 1-101; Coverage: 100%)
Gene names: rps6, rpsF
Sequence domains: Ribosomal protein S6
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6422
Unit cell:
a: 84.252Å b: 84.252Å c: 144.367Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.236
Expression system: Escherichia coli

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Citations

3 citation in other articles

Competition between native topology and nonnative interactions in simple and complex folding kinetics of natural and designed proteins.
Zhang et al. (2010)
2 more