› 2bzd

X-ray diffraction
2Å resolution

Galactose recognition by the carbohydrate-binding module of a bacterial sialidase.

Released:
DOI: 10.2210/pdb2bzd/pdb
PDB entry 2bzd coloured by chain, front view.
PDB entry 2bzd coloured by chain, side view.
PDB entry 2bzd coloured by chain, top view.
Source organism: Micromonospora viridifaciens
Primary publication:
Galactose recognition by the carbohydrate-binding module of a bacterial sialidase.
Newstead SL, Watson JN, Bennet AJ, Taylor G
Acta Crystallogr D Biol Crystallogr 61 1483-91 (2005)
PMID: 16239725

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 601 amino acids
Theoretical weight: 64.24 KDa
Source organism: Micromonospora viridifaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02834 (Residues: 47-647; Coverage: 99%)
Gene name: nedA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand GAL 3 x GAL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3221
Unit cell:
a: 141.457Å b: 141.457Å c: 158.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.193 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Insights into the recognition of the human glycome by microbial carbohydrate-binding modules.
Ficko-Blean et al. (2012)
26 other articles

3 mentions without citation

Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2.
Klontz et al. (2019)
2 more