› 2c19

X-ray diffraction
2.05Å resolution

5-(4-Carboxy-2-oxo-butylsulfanyl)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2c19/pdb
PDB entry 2c19 coloured by chain, front view.
PDB entry 2c19 coloured by chain, side view.
PDB entry 2c19 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors.
Frère F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N
Biochemistry 45 8243-53 (2006)
PMID: 16819823

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.29 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59643 (Residues: 1-337; Coverage: 100%)
Gene names: PA5243, hemB
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand NA 2 x NA
1 modified residue:
Ligand C1X 2 x C1X

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4212
Unit cell:
a: 127.203Å b: 127.203Å c: 85.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.144 0.201
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

The biochemistry of heme biosynthesis.
Heinemann et al. (2008)
1 more

73 mentions without citation

In vitro evaluation of reversible and irreversible cytochrome P450 inhibition: current status on methodologies and their utility for predicting drug-drug interactions.
Fowler et al. (2008)
72 more