2c29

X-ray diffraction
1.81Å resolution

Structure of dihydroflavonol reductase from Vitis vinifera at 1.8 A.

Released:

Function and Biology Details

Reactions catalysed:
A (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH
(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavanone 4-reductase Chains: D, F
Molecule details ›
Chains: D, F
Length: 337 amino acids
Theoretical weight: 37.69 KDa
Source organism: Vitis vinifera
Expression system: Escherichia coli
UniProt:
  • Canonical: P93799 (Residues: 1-337; Coverage: 100%)
Gene name: dfr1
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 87.818Å b: 89.927Å c: 93.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.244
Expression system: Escherichia coli