› 2c2v

X-ray diffraction
2.9Å resolution

Crystal structure of the CHIP-UBC13-UEV1a complex

Released:
DOI: 10.2210/pdb2c2v/pdb
PDB entry 2c2v coloured by chain, front view.
PDB entry 2c2v coloured by chain, side view.
PDB entry 2c2v coloured by chain, top view.
Source organisms:
Primary publication:
Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.
Zhang M, Windheim M, Roe SM, Peggie M, Cohen P, Prodromou C, Pearl LH
Mol Cell 20 525-38 (2005)
PMID: 16307917

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-158217 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin-conjugating enzyme E2 N Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 154 amino acids
Theoretical weight: 17.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61088 (Residues: 2-152; Coverage: 99%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Ubiquitin-conjugating enzyme E2 variant 1 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 142 amino acids
Theoretical weight: 16.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13404 (Residues: 7-147; Coverage: 96%)
Gene names: CROC1, P/OKcl.19, UBE2V, UBE2V1, UEV1
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
E3 ubiquitin-protein ligase CHIP Chains: S, T, U, V
Molecule details ›
Chains: S, T, U, V
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WUD1 (Residues: 227-304; Coverage: 26%)
Gene names: Chip, Stub1
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 180.31Å b: 69.991Å c: 204.477Å
α: 90° β: 106.95° γ: 90°
R-values:
R R work R free
0.218 0.214 0.297
Expression system: Escherichia coli

Quick links

Citations

68 review citations

The ubiquitin code.
Komander et al. (2012)
67 more

24 mentions without citation

Building ubiquitin chains: E2 enzymes at work.
Ye et al. (2009)
23 more