› 2c3f

X-ray diffraction
1.81Å resolution

The structure of a group A streptococcal phage-encoded tail-fibre showing hyaluronan lyase activity.

Released:
DOI: 10.2210/pdb2c3f/pdb
PDB entry 2c3f coloured by chain, front view.
PDB entry 2c3f coloured by chain, side view.
PDB entry 2c3f coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix.
Smith NL, Taylor EJ, Lindsay AM, Charnock SJ, Turkenburg JP, Dodson EJ, Davies GJ, Black GW
Proc Natl Acad Sci U S A 102 17652-7 (2005)
PMID: 16314578

Function and Biology Details

Reaction catalysed: 
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major tropism determinant N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 38.59 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9A0M7 (Residues: 2-337; Coverage: 100%)
Gene names: SPy_0701, hylP1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 58.534Å b: 58.534Å c: 586.585Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
13 more

4 mentions without citation

Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix.
Smith et al. (2005)
3 more