› 2c8i

Electron Microscopy
14Å resolution

Complex Of Echovirus Type 12 With Domains 1, 2, 3 and 4 Of Its Receptor Decay Accelerating Factor (Cd55) By Cryo Electron Microscopy At 16 A

Released:
DOI: 10.2210/pdb2c8i/pdb
PDB entry 2c8i coloured by chain, front view.
PDB entry 2c8i coloured by chain, side view.
PDB entry 2c8i coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional insights into the interaction of echoviruses and decay-accelerating factor.
Pettigrew DM, Williams DT, Kerrigan D, Evans DJ, Lea SM, Bhella D
J Biol Chem 281 5169-77 (2006)
PMID: 16272562
Related structures: EMD-1183

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139972 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.45 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: P29813 (Residues: 570-858; Coverage: 13%)
Sequence domains: picornavirus capsid protein
Capsid protein VP2 Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: P29813 (Residues: 79-330; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 25.9 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: P29813 (Residues: 332-569; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 60 amino acids
Theoretical weight: 6.62 KDa
Source organism: Echovirus E11
UniProt:
  • Canonical: P29813 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Complement decay-accelerating factor Chain: E
Molecule details ›
Chain: E
Length: 316 amino acids
Theoretical weight: 35.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08174 (Residues: 35-285; Coverage: 72%)
Gene names: CD55, CR, DAF
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 14Å
Relevant EMDB volumes: EMD-1183
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Blood Groups in Infection and Host Susceptibility.
Cooling L. (2015)
4 more

2 mentions without citation

The role of cellular adhesion molecules in virus attachment and entry.
Bhella D. (2015)
1 more