› 2c98

X-ray diffraction
1.9Å resolution

Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF

Released:
DOI: 10.2210/pdb2c98/pdb
PDB entry 2c98 coloured by chain, front view.
PDB entry 2c98 coloured by chain, side view.
PDB entry 2c98 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF.
Rappas M, Schumacher J, Niwa H, Buck M, Zhang X
J Mol Biol 357 481-92 (2006)
PMID: 16430918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Psp operon transcriptional activator Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37344 (Residues: 1-265; Coverage: 82%)
Gene names: JW1296, b1303, pspF, ycjB
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P65
Unit cell:
a: 113.097Å b: 113.097Å c: 39.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.161 0.203
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Managing membrane stress: the phage shock protein (Psp) response, from molecular mechanisms to physiology.
Joly et al. (2010)
10 more

6 mentions without citation

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
5 more