› 2cai

X-ray diffraction
2.26Å resolution

Structure of Glutathione-S-Transferase mutant, R21L, from Schistosoma Haematobium

Released:
DOI: 10.2210/pdb2cai/pdb
PDB entry 2cai coloured by chain, front view.
PDB entry 2cai coloured by chain, side view.
PDB entry 2cai coloured by chain, top view.
Source organism: Schistosoma haematobium
Primary publication:
Probing the mechanism of GSH activation in Schistosoma haematobium glutathione-S-transferase by site-directed mutagenesis and X-ray crystallography.
Baiocco P, Gourlay LJ, Angelucci F, Fontaine J, Hervé M, Miele AE, Trottein F, Brunori M, Bellelli A
J Mol Biol 360 678-89 (2006)
PMID: 16777141

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 28 kDa isozyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.89 KDa
Source organism: Schistosoma haematobium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30113 (Residues: 1-211; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 10 x SO4
Ligand PG4 3 x PG4
Ligand BME 3 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I23
Unit cell:
a: 148.716Å b: 148.716Å c: 148.716Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Glutathione transferases from parasites: a biochemical view.
Torres-Rivera et al. (2008)
2 more

1 mention without citation

Molecular Characterization of Coxsackievirus B5 Isolates from Sewage, Italy 2016-2017.
Fontana et al. (2019)