› 2cax

X-ray diffraction
2.9Å resolution

STRUCTURAL BASIS FOR COOPERATIVE BINDING OF RIBBON-HELIX-HELIX REPRESSOR OMEGA TO MUTATED DIRECT DNA HEPTAD REPEATS

Released:
DOI: 10.2210/pdb2cax/pdb
PDB entry 2cax coloured by chain, front view.
PDB entry 2cax coloured by chain, side view.
PDB entry 2cax coloured by chain, top view.
Source organisms:
Primary publication:
Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription.
Weihofen WA, Cicek A, Pratto F, Alonso JC, Saenger W
Nucleic Acids Res 34 1450-8 (2006)
PMID: 16528102

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115145 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Omega transcriptional repressor domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 53 amino acids
Theoretical weight: 6.14 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q57468 (Residues: 20-71; Coverage: 73%)
Gene name: omega
Sequence domains: Omega Transcriptional Repressor
Structure domains: Met repressor-like
5'-D(*GP*AP*AP*TP*CP*AP*CP*AP*AP*AP *TP*CP*AP*CP*AP*AP*G)-3' Chain: G
Molecule details ›
Chain: G
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
5'-D(*TP*TP*GP*TP*GP*AP*TP*TP*TP*GP *TP*GP*AP*TP*TP*CP*G)-3' Chain: H
Molecule details ›
Chain: H
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*GP*AP*AP*TP*CP*AP*CP*AP*AP*GP *TP*CP*AP*CP*AP*AP*GP*C)-3' Chain: U
Molecule details ›
Chain: U
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
5'-D(*CP*TP*TP*GP*TP*GP*AP*CP*TP*TP *GP*TP*GP*AP*TP*TP*CP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 18 nucleotides
Theoretical weight: 5.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 219.957Å b: 44.655Å c: 76.136Å
α: 90° β: 109.26° γ: 90°
R-values:
R R work R free
0.21 0.208 0.247
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

8 review citations

To be or not to be: regulation of restriction-modification systems and other toxin-antitoxin systems.
Mruk et al. (2014)
7 more

4 mentions without citation

Structures of omega repressors bound to direct and inverted DNA repeats explain modulation of transcription.
Weihofen et al. (2006)
3 more