› 2cff

X-ray diffraction
2.5Å resolution

Crystal Structure Of N-((5'-Phosphoribosyl)-Formimino)-5- Aminoimidazol-4-Carboxamid Ribonucleotid Isomerase mutant D127V (Ec 3. 1.3.15, Hisa)

Released:
DOI: 10.2210/pdb2cff/pdb
PDB entry 2cff coloured by chain, front view.
PDB entry 2cff coloured by chain, side view.
PDB entry 2cff coloured by chain, top view.
Source organism: Thermotoga maritima
Entry authors: He C, Kuper J, Sterner R, Wilmmans M

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.05 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0C7 (Residues: 1-241; Coverage: 100%)
Gene names: TM_1037, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 48.71Å b: 47.08Å c: 105.25Å
α: 90° β: 98.1° γ: 90°
R-values:
R R work R free
0.2 0.2 0.283
Expression system: Escherichia coli

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