› 2chg

X-ray diffraction
2.1Å resolution

Replication Factor C domains 1 and 2

Released:
DOI: 10.2210/pdb2chg/pdb
PDB entry 2chg coloured by chain, front view.
PDB entry 2chg coloured by chain, side view.
PDB entry 2chg coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Communication between subunits within an archaeal clamp-loader complex.
Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB
EMBO J 25 2209-18 (2006)
PMID: 16628222

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication factor C small subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 226 amino acids
Theoretical weight: 25.38 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28219 (Residues: 1-226; Coverage: 71%)
Gene names: AF_2060, rfcS
Sequence domains: ATPase family associated with various cellular activities (AAA)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 77.504Å b: 91.472Å c: 140.644Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.256
Expression system: Escherichia coli

Quick links

Citations

6 review citations

DNA replication in the archaea.
Barry et al. (2006)
5 more

2 mentions without citation

Fundamental Characteristics of AAA+ Protein Family Structure and Function.
Miller et al. (2016)
1 more