› 2chn

X-ray diffraction
1.95Å resolution

Bacteroides thetaiotaomicron hexosaminidase with O-GlcNAcase activity- NAG-thiazoline complex

Released:
DOI: 10.2210/pdb2chn/pdb
PDB entry 2chn coloured by chain, front view.
PDB entry 2chn coloured by chain, side view.
PDB entry 2chn coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity.
Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ
Nat Struct Mol Biol 13 365-71 (2006)
PMID: 16565725

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162662 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
O-GlcNAcase BT_4395 Chains: A, B
Molecule details ›
Chains: A, B
Length: 716 amino acids
Theoretical weight: 83.08 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q89ZI2 (Residues: 22-737; Coverage: 100%)
Gene name: BT_4395
Sequence domains:
Structure domains:
GLUCOSAMINIDASE Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand NGT 2 x NGT
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 51.714Å b: 94.544Å c: 99.479Å
α: 75.34° β: 93.86° γ: 77.07°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

33 review citations

Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease.
Hart et al. (2011)
32 more

10 mentions without citation

Sculpting neural circuits by axon and dendrite pruning.
Riccomagno et al. (2015)
9 more