› 2cjs

X-ray diffraction
1.78Å resolution

Structural Basis for a Munc13-1 Homodimer - Munc13-1 - RIM Heterodimer Switch: C2-domains as Versatile Protein-Protein Interaction Modules

Released:
DOI: 10.2210/pdb2cjs/pdb
PDB entry 2cjs coloured by chain, front view.
PDB entry 2cjs coloured by chain, side view.
PDB entry 2cjs coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch.
Lu J, Machius M, Dulubova I, Dai H, Südhof TC, Tomchick DR, Rizo J
PLoS Biol 4 e192 (2006)
PMID: 16732694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178969 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein unc-13 homolog A Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q62768 (Residues: 1-150; Coverage: 9%)
Gene names: Unc13a, Unc13h1
Sequence domains: C2 domain
Structure domains: C2 domain
Regulating synaptic membrane exocytosis protein 2 Chain: C
Molecule details ›
Chain: C
Length: 62 amino acids
Theoretical weight: 6.87 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9JIS1 (Residues: 82-142; Coverage: 4%)
Gene names: Rim2, Rims2
Sequence domains: FYVE-type zinc finger
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand GOL 6 x GOL
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 50.248Å b: 93.534Å c: 113.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

23 review citations

Neurotransmitter release: the last millisecond in the life of a synaptic vesicle.
Südhof TC. (2013)
22 more

7 mentions without citation

Mechanism of neurotransmitter release coming into focus.
Rizo J. (2018)
6 more