› 2cks

X-ray diffraction
1.6Å resolution

X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5)

Released:
DOI: 10.2210/pdb2cks/pdb
PDB entry 2cks coloured by chain, front view.
PDB entry 2cks coloured by chain, side view.
PDB entry 2cks coloured by chain, top view.
Source organism: Thermobifida fusca
Entry authors: Berglund GI, Gualfetti PJ, Requadt C, Gross LS, Bergfors T, Shaw A, Saldajeno M, Mitchinson C, Sandgren M

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase E-5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.18 KDa
Source organism: Thermobifida fusca
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q01786 (Residues: 161-466; Coverage: 71%)
Gene name: celE
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand BEN 2 x BEN
Ligand ZN 8 x ZN
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 48.888Å b: 70.97Å c: 75.624Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.142 0.142 0.169
Expression system: Bacillus subtilis

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