› 2cnu

X-ray diffraction
1.05Å resolution

Atomic resolution structure of SAICAR-synthase from Saccharomyces cerevisiae complexed with aspartic acid

Released:
DOI: 10.2210/pdb2cnu/pdb
PDB entry 2cnu coloured by chain, front view.
PDB entry 2cnu coloured by chain, side view.
PDB entry 2cnu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Urusova DV, Antonyuk SV, Grebenko AI, Levdikov VM, Barynin VV, Popov AN, Lamzin VS, Melik-Adamyan WR

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.55 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P27616 (Residues: 2-306; Coverage: 100%)
Gene names: ADE1, YAR015W
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ASP 3 x ASP
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 61.023Å b: 62.497Å c: 78.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.159

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