› 2co0

X-ray diffraction
2.25Å resolution

WDR5 and unmodified Histone H3 complex at 2.25 Angstrom

Released:
DOI: 10.2210/pdb2co0/pdb
PDB entry 2co0 coloured by chain, front view.
PDB entry 2co0 coloured by chain, side view.
PDB entry 2co0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex.
Ruthenburg AJ, Wang W, Graybosch DM, Li H, Allis CD, Patel DJ, Verdine GL
Nat Struct Mol Biol 13 704-12 (2006)
PMID: 16829959

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158475 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61964 (Residues: 20-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3.1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 15 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-16; Coverage: 11%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
WD repeat-containing protein 5 Chain: C
Molecule details ›
Chain: C
Length: 315 amino acids
Theoretical weight: 34.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61964 (Residues: 20-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU R-AXIS IV
Spacegroup: P21
Unit cell:
a: 64.916Å b: 47.03Å c: 101.768Å
α: 90° β: 107.01° γ: 90°
R-values:
R R work R free
0.19 0.19 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

46 review citations

Chromatin modifications and their function.
Kouzarides T. (2007)
45 more

5 mentions without citation

Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex.
Ruthenburg et al. (2006)
4 more