› 2d06

X-ray diffraction
2.3Å resolution

Human Sult1A1 Complexed With Pap and estradiol

Released:
DOI: 10.2210/pdb2d06/pdb
PDB entry 2d06 coloured by chain, front view.
PDB entry 2d06 coloured by chain, side view.
PDB entry 2d06 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human SULT1A1 crystallized with estradiol. An insight into active site plasticity and substrate inhibition with multi-ring substrates.
Gamage NU, Tsvetanov S, Duggleby RG, McManus ME, Martin JL
J Biol Chem 280 41482-6 (2005)
PMID: 16221673

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 34.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50225 (Residues: 1-295; Coverage: 100%)
Gene names: OK/SW-cl.88, STP, STP1, SULT1A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand A3P 2 x A3P
Ligand EST 2 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 123.64Å b: 86.07Å c: 72.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.453 0.217 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Enzyme promiscuity: a mechanistic and evolutionary perspective.
Khersonsky et al. (2010)
16 more

15 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
14 more