› 2d7c

X-ray diffraction
1.75Å resolution

Crystal structure of human Rab11 in complex with FIP3 Rab-binding domain

Released:
DOI: 10.2210/pdb2d7c/pdb
PDB entry 2d7c coloured by chain, front view.
PDB entry 2d7c coloured by chain, side view.
PDB entry 2d7c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1.
Shiba T, Koga H, Shin HW, Kawasaki M, Kato R, Nakayama K, Wakatsuki S
Proc Natl Acad Sci U S A 103 15416-21 (2006)
PMID: 17030804

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130858 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-11A Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62491 (Residues: 7-173; Coverage: 77%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rab11 family-interacting protein 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 42 amino acids
Theoretical weight: 5.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O75154 (Residues: 715-756; Coverage: 6%)
Gene names: ARFO1, KIAA0665, RAB11FIP3
Sequence domains: FIP domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
Ligand MES 1 x MES
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A, PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 108.717Å b: 120.565Å c: 36.249Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Cell biology and physiology of the uroepithelium.
Khandelwal et al. (2009)
11 more

4 mentions without citation

Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1.
Shiba et al. (2006)
3 more