2d7h

X-ray diffraction
3Å resolution

Crystal structure of the ccc complex of the N-terminal domain of PriA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-115161 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Primosomal protein N' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17888 (Residues: 1-105; Coverage: 14%)
Gene names: JW3906, b3935, priA
Sequence domains: 3'DNA-binding domain (3'BD)
Structure domains: PriA, 3(prime) DNA-binding domain
DNA (5'-D(P*CP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 3 nucleotides
Theoretical weight: 823 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R32
Unit cell:
a: 111.151Å b: 111.151Å c: 260.483Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.238 0.293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided