› 2dkf

X-ray diffraction
2.8Å resolution

Crystal Structure of TTHA0252 from Thermus thermophilus HB8, a RNA Degradation Protein of the Metallo-beta-lactamase Superfamily

Released:
DOI: 10.2210/pdb2dkf/pdb
PDB entry 2dkf coloured by chain, front view.
PDB entry 2dkf coloured by chain, side view.
PDB entry 2dkf coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of TTHA0252 from Thermus thermophilus HB8, a RNA degradation protein of the metallo-beta-lactamase superfamily.
Ishikawa H, Nakagawa N, Kuramitsu S, Masui R
J Biochem 140 535-42 (2006)
PMID: 16945939

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-178188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease TTHA0252 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.45 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 143.22Å b: 147.1Å c: 121.23Å
α: 90° β: 109.25° γ: 90°
R-values:
R R work R free
0.242 0.242 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
7 more

2 mentions without citation

SPLINTS: small-molecule protein ligand interface stabilizers.
Fischer et al. (2016)
1 more