› 2dpg

X-ray diffraction
2.5Å resolution

COMPLEX OF INACTIVE MUTANT (H240->N) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES WITH NADP+

Released:
DOI: 10.2210/pdb2dpg/pdb
PDB entry 2dpg coloured by chain, front view.
PDB entry 2dpg coloured by chain, side view.
PDB entry 2dpg coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
On the mechanism of the reaction catalyzed by glucose 6-phosphate dehydrogenase.
Cosgrove MS, Naylor C, Paludan S, Adams MJ, Levy HR
Biochemistry 37 2759-67 (1998)
PMID: 9485426

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NAD(P)(+) = 6-phospho-D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 54.35 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: P11411 (Residues: 2-486; Coverage: 100%)
Gene name: zwf
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P6222
Unit cell:
a: 136.7Å b: 136.7Å c: 121.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.173 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular chaperones are nanomachines that catalytically unfold misfolded and alternatively folded proteins.
Mattoo et al. (2014)
2 more