› 2dqx

X-ray diffraction
2.2Å resolution

mutant beta-amylase (W55R) from soy bean

Released:
DOI: 10.2210/pdb2dqx/pdb
PDB entry 2dqx coloured by chain, front view.
PDB entry 2dqx coloured by chain, side view.
PDB entry 2dqx coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Kinetic and structural analysis of enzyme sliding on a substrate: multiple attack in beta-amylase.
Ishikawa K, Nakatani H, Katsuya Y, Fukazawa C
Biochemistry 46 792-8 (2007)
PMID: 17223700

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-amylase Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 55.97 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: Q42795 (Residues: 2-496; Coverage: 100%)
Gene names: 547931, GLYMA_06G301500
Sequence domains: Glycosyl hydrolase family 14
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P3121
Unit cell:
a: 85.042Å b: 85.042Å c: 145.083Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.252
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Starch-degrading polysaccharide monooxygenases.
Vu et al. (2016)
3 other articles