› 2dsq

X-ray diffraction
2.8Å resolution

Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins

Released:
DOI: 10.2210/pdb2dsq/pdb
PDB entry 2dsq coloured by chain, front view.
PDB entry 2dsq coloured by chain, side view.
PDB entry 2dsq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the inhibition of insulin-like growth factors by insulin-like growth factor-binding proteins.
Sitar T, Popowicz GM, Siwanowicz I, Huber R, Holak TA
Proc Natl Acad Sci U S A 103 13028-33 (2006)
PMID: 16924115

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-140390 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin-like growth factor-binding protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 9.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 22-113; Coverage: 39%)
Gene names: IBP4, IGFBP4
Sequence domains: Insulin-like growth factor binding protein
Structure domains: Omega-AgatoxinV
Insulin-like growth factor I Chains: C, I
Molecule details ›
Chains: C, I
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NP10 (Residues: 26-95; Coverage: 67%)
Gene name: IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like
Insulin-like growth factor-binding protein 1 Chains: G, H
Molecule details ›
Chains: G, H
Length: 94 amino acids
Theoretical weight: 10.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08833 (Residues: 166-259; Coverage: 40%)
Gene names: IBP1, IGFBP1
Sequence domains: Thyroglobulin type-1 repeat
Structure domains: Thyroglobulin type-1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 71.28Å b: 43.66Å c: 81.15Å
α: 90° β: 91.67° γ: 90°
R-values:
R R work R free
0.292 0.288 0.357
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

IGF binding proteins in cancer: mechanistic and clinical insights.
Baxter RC. (2014)
26 more

7 mentions without citation

Defining the pathway to insulin-like growth factor system targeting in cancer.
Rosenzweig et al. (2010)
6 more