› 2e37

X-ray diffraction
2.3Å resolution

Structure of TT0471 protein from Thermus thermophilus

Released:
DOI: 10.2210/pdb2e37/pdb
PDB entry 2e37 coloured by chain, front view.
PDB entry 2e37 coloured by chain, side view.
PDB entry 2e37 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Lokanath NK, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 310 amino acids
Theoretical weight: 32.97 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJA1 (Residues: 1-310; Coverage: 100%)
Gene names: TTHA1113, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 162.74Å b: 135.638Å c: 162.056Å
α: 90° β: 113.79° γ: 90°
R-values:
R R work R free
0.215 0.215 0.255
Expression system: Escherichia coli BL21(DE3)

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